| USP6NL

USP6 N-terminal-like protein is a protein that is encoded by the USP6NL gene.Enables GTPase activator activity and small GTPase binding activity. Involved in several processes, including plasma membrane to endosome transport; positive regulation of GTPase activity; and retrograde transport, plasma membrane to Golgi. Located in cytoplasmic vesicle and plasma membrane.USP6NL is a Protein Coding gene. Among its related pathways are Vesicle-mediated transport and PDGFR-beta signaling pathway.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.26	1.45	0.85	0.55	0.36	0.27	0.19	0.07
Corrected OD	2.19	1.38	0.78	0.48	0.29	0.2	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human USP6NL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	90-105	100

 

| Linearity

Samples were spiked with high concentrations of Human USP6NL and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)
1 : 2	Range (%)	85-96
	Average (%)	93
1 : 4	Range (%)	87-108
	Average (%)	102